貨號 | 名稱 | 規格 |
CG1120A | Activated Charcoal Absorbed（碳吸附胎牛血清） | 500ml |
CG1120B | Activated Charcoal Absorbed（碳吸附胎牛血清） | 50ml×10 |
Description
Fetal Bovine Serum (FBS) Activated Charcoal Absorbed is produced by filtering FBS through an activated carbon-absorbent filter, resulting in reduced concentrations of steroid hormones, such as estradiol, progesterone, cortisol, testosterone, T3, and T4, without unspecific loss of other serum components. FBS Charcoal Stripped is useful for studying processes influenced by steroid hormones (estrogen stimulation, obesity process) and other research topics that benefit from reduced hormone levels, such as certain types of viral infections.
Fetal bovine serum (FBS) or fetal calf serum (FCS) is widely used in eukaryotic cell culture, typically as a supplement for growth media. Fetal bovine serum contains nutritional and embryonic growth promoting factors that are essential for a successful cell culture. Further, fetal bovine serum contains various small molecules, such as, e.g., amino acids, lipids, sugars, and hormones.
Fetal bovine serum (FBS) is the blood component obtained after coagulation and removal of cellular components. Its unique and comprehensive mix makes FBS one of the most important supplements for supporting cell growth and proliferation for in vitro cell culture.
Our fetal bovine serum for cell culture supports most cell lines and undergoes strict quality controls.
【產品介紹】
常規細胞培養需要添加血清來維持細胞的增殖能力，盡管血清可以滿足一般細胞培養需求，但在進行營養成分優化和標記特定成分進行研究的實驗中，仍需要去除某些特定的背景成分，常用的去除方法有碳吸附。
碳吸附胎牛血清用于體外培養，去除背景值以驗證激素的作用效果，研究包括類固醇受體連接，細胞受體類固醇調節，各種組織激素分泌和甲狀腺激素功能。生產過程中，使用炭和右旋糖苷去除血清中的激素。
血清通常用作細胞生長的添加物，擁有多種功能和用途，如：富含大分子蛋白，低分子量的營養素，作為氨基酸、碳水化合物、維生素的額外來源；提高介質的緩沖能力；可結合或中和有毒成分；減少細胞氧化應激，減少細胞凋亡；提供促進生長的因子，有助細胞適應培養環境。
【適用范圍】
碳吸附胎牛血清常用于體外培養研究驗證激素的作用效果。
適用于：培養干細胞、原代細胞、神經細胞、人類癌細胞、人類骨髓細胞、白血病細胞、人類胚肺成纖維細胞、人類腦神經細胞和瘤細胞等細胞。
【使用方法】
血清從冰凍區中取出后（切勿直接將血清從-20℃進入37℃解凍，這樣因溫度改變太大，容易造成蛋白質凝集而出現沉淀），置于2℃～8℃中使之融化，解凍過程中間歇地輕輕搖晃均勻，待全部溶解后再分裝或直接使用。完全融解的血清從4℃拿出，按要求比例（5%-20%）加基礎培養基配置成完全培養基，建議其置于4℃冰箱存放并于1-2周內用完。