Zymo Research – DNA甲基化分析的完整解決方案
Zymo于1994年成立于美國加州Orange County,主要研發和生產表觀遺傳學系列產品和常用分子生物學試劑盒,其DNA甲基化,羥甲基化,核酸提取等產品已得到世界各頂級實驗室的認可,產品具有“快速、簡便、超純”的特點;
Zymo甲基化產品線已被上千次引用在各大主流學術刊物上,包括NATURE、SCIENCE 、PNAS、Stem Cells、 Nucleic Acids Res等;迄今,EZ DNA Methylation?系列產品作為DNA重亞硫酸鹽處理(DNA甲基化分析前處理)的的金標準,已成為最暢銷和引用次數最多的產品;
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產品 |
規格 |
貨號 |
報價 |
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EZ DNA Methylation-Gold? Kit |
50 Rxns |
D5005 |
2117 |
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EZ DNA Methylation-Gold? Kit |
200 Rxns |
D5006 |
7109 |
產品介紹:
- 富含GC的DNA亞硫酸鹽轉化過程少于3小時
- 熱變性/轉化反應簡化了非甲基化的胞嘧啶轉化為尿嘧啶的過程
- 采用離心柱對硫酸鹽處理的DNA進行脫硫化(desulphonation)和回收
- 回收的DNA可用于PCR、內切酶消化、測序、芯片分析等
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產品介紹 |
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The EZ DNA Methylation-Gold? Kit is a refinement of our popular EZ DNA Methylation? kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. |
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產品特點 |
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形式: |
Spin Column |
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DNA回收率: |
> 75% |
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實驗時間: |
3 hrs |
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設備: |
Microcentrifuge |
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結合能力: |
5 μg/prep |
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洗脫體積: |
≥ 10 μl |
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轉化率: |
> 99% |
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樣品: |
Plasmid, genomic, and endonuclease digested DNAs. |
相關文獻
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D5005: |
