Epicypher新品 | 核酸提取緩沖液
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CUTANA? 細胞核提取緩沖液是CUT&RUN和CUT&Tag實驗中，用于從培養(yǎng)的細胞和組織中提取細胞核的必需試劑。這種緩沖液可與多種真核細胞和組織兼容。以下是使用該緩沖液成功進行細胞核分離的細胞和組織類型：
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mouse NIH3T3 fibroblast cells |
human A549 non-small cell lung cancer (NSCLC) cells |
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human K562 leukemia cells |
human NCI-H1299 non-small cell lung cancer (NSCLS cells) |
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human bone marrow derived macrophages |
human TIG-1 fetal lung cells |
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human monocyte derived macrophages |
human LoVo colorectal cancer cells |
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human MV-4-11 macrophage cells |
human LNCaP prostate carcinoma cells |
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human SUM149 triple negative breast cancer (TNBC) cells |
human renal primary cells |
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human GM24385 B-lymphocyte (aka HG002) cells |
human peripheral blood mononuclear cells (PBMCs) |
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human MCF7 breast cancer cells |
human intestinal tissue |
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human MDA-MB-231 breast cancer cells |
human HEPM embryonic cells |
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human SK-MEL-2 melanoma cells |
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只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA? 細胞核提取緩沖液針對CUT&RUN和CUT&Tag實驗流程進行了優(yōu)化，從而獲得高質(zhì)量的細胞核，助力您的基因組圖譜分析。
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試劑盒組成
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組分 |
貨號 |
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CUTANA??Pre-Nuclei Extraction Buffer? ? ?? |
21-1026a? ? ? |
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1 M Spermidine |
21-1026b |
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保存條件
將Pre-Nuclei Extraction Buffer 4oC儲存, 1 M亞精胺-20oC儲存。
自收到之日起可穩(wěn)定儲存6個月。
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驗證數(shù)據(jù)
Figure 1: Nuclei Extraction
Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA? Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA? Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.
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訂購詳情
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貨號 |
產(chǎn)品名稱 |
規(guī)格 |
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21-1026 |
CUTANA? Nuclei Extraction Buffer |
100 Reactions |
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