
High-OxidizedLDL高氧化型低密度脂蛋白
產(chǎn)品名稱: High-OxidizedLDL高氧化型低密度脂蛋白
英文名稱:
產(chǎn)品編號(hào): H7980-4mg
產(chǎn)品價(jià)格: 0
產(chǎn)品產(chǎn)地: 中國(guó) 北京索萊寶
品牌商標(biāo): solarbio
更新時(shí)間: 2025-09-16T09:00:33
使用范圍: null
- 聯(lián)系人 : 索萊寶-龔思雨
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High-Oxidized Low Density Lipoprotein, Human
Cat?No:?H7980
Size: 2.0mg(milligrams)Protein/vial
Concentration:?>1mg/ml?protein?(Based on actual label concentration)
Specifications: High Oxidized LDL is membrane filtered and aseptically packaged in a solution
containing phosphate-buffered saline at pH 7.4.
Storage:
This product is stable for only 3?weeks?after receipt when handled aseptically and stored at 2-8°C (Do not freeze). After prolonged storage, some precipitate may be observed. This is normal for this product. Spin in a centrifugation 1000 x g for 3 minutes before using.
Biological?Activity:
Sample lots of Oxidized LDL are evaluated for receptor binding to?peritoneal macrophages in conjunction with our DiI-Ox-LDL and [I-125] Ox-LDL
Preparation: ?????
Human LDL (which was purified to homogeneity via ultra?centrifugation(1.019-1.063g/cc)) is extensively oxidized with Cu2SO4?(oxidant) in PBS at 37°C. Oxidation is terminated by adding excess EDTA-Na2. ?Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of Ox-LDL migrates 3.0fold further than the native LDL.?This product can produce potent oxidative stress and can be used to induce cell apoptosis/death (>50%) and produce cell injure model.
TBARS:
TBARS is determined colorimetrically by using Malondialdehyde as a standard.
Starting??LDL ??????0.5±0.5 ??nmoles?of??MDA/mg Protein
High?????Ox-LDL ??90.0±9.9 nmoles?of ?MDA/mg Protein???
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