HisPrep FF 16/10
產品名稱： HisPrep FF 16/10
英文名稱： HisPrep FF 16/10
產品編號： 28-9365-51
產品價格： 0
產品產地： 美國
品牌商標： GE
更新時間： null
使用范圍： null
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| 28-9365-51 | HisPrep FF 16/10 | 1 ×20 ml |
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* Pack size available by special order.
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HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90-μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
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HisPrep? FF 16/10 column for convenient scale-up purification of histidine-tagged proteins.
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Customer feedback on Ni Sepharose 6 Fast Flow.
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* For licensing information, see Legal Info.
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HiPrep IMAC FF 16/10, see IMAC Sepharose 6 Fast Flow Media/HiTrap IMAC FF Columns/HiPrep IMAC FF 16/10 Column/HiScreen IMAC FF Column, is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
For the complete range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.
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HisPrep? FF 16/10 Column - Easy Scale-up Purification
Technical Information
| TECHNICAL SPECIFICATIONS | |
| Medium? | Ni Sepharose 6 Fast Flow? |
| Column volume? | 20 ml? |
| Dynamic binding?capacity*? | Approx. 40 mg histidine-tagged protein/ml medium? |
| Column dimensions? | 1.6 × 10 cm? |
| Recommended flow rate?? | 2-10 ml/min (60-300 cm/h)? |
| Max. flow rate?? | 10 ml/min (300 cm/h)? |
| Max. pressure over the packed bed during operation, Dp? | 0.15 MPa, 1.5 bar? |
| Column hardware pressure limit? | 0.5 MPa, 5 bar? |
| Compatibility? | Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information.? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
| * Binding capacity is protein-to-protein dependent ? H2O at room temperature |
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