Chlamydia trachomatis LGV-II (434 Strain) Quantitated DNA
產品名稱： Chlamydia trachomatis LGV-II (434 Strain) Quantitated DNA
英文名稱： Chlamydia trachomatis LGV-II (434 Strain) Quantitated DNA
產品編號： Chlamydia trachomatis LGV-II (434 Strain) Qua
產品價格： 0
產品產地： 美國
品牌商標：
更新時間： 2023-09-19T20:47:01
使用范圍： null
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| Description(名稱） | Chlamydia trachomatis LGV-II (434 Strain) Quantitated DNA |
| Catalog #（貨號） | 08-931-000 |
| ?Size（規格） | 250?μL |
| ?Price（價格） | ??請聯系：0769-2289-0558， 0769-22890598， QQ:2533190771 |
???????????????????????????????????????????? Description（產品描述）：?
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Chlamydia trachomatis?serovar L2 (434 strain) Quantitated DNA PCR control.
For research use only. Not for use in diagnostic procedures.
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ABI’s Quantitated PCR Controls can be used to generate standard curves for quantitative PCR assays and as positive controls in nucleic acid ampli?cation reactions. Quantitated PCR Controls for viruses, bacteria, and other organisms such as?Toxoplasma gondii?and?Mycoplasma pneumoniae?are available.
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ABI’s Quantitated PCR Controls are purified from one of the following sources:
- Intact Viral Particles, Parasites or Mollicutes
- Cultured Bacteria: pelleted bacteria from liquid, plate, or slant culture
- Cloned viral DNA
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Quantitated PCR Controls are supplied in volumes of 250 μL at 1-2 x 104?DNA copies/μL (except for BK and JC polyomaviruses, and Porcine Circovirus Type 2 (PCV2), which are supplied at 1-2 x 105?DNA copies/μL). Quantitation with different instruments and by different methods may not give the same copy number results.
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Note: ABI’s Quantitated PCR Controls are intended for use as positive PCR quantitative standards for the organism in question. Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls. Additionally, due to the extreme sensitivity of detection in PCR reactions, and since no method of puri?cation can guarantee the complete absence of extraneous agents, PCR controls are not intended for use as negative controls for other organisms.
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ABI’s PCR Controls are for research use only and are not for use in diagnostic assays.
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Details（注意事項）:
Shipping and Storage:?This product is shipped frozen on dry ice.?Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Recommendations:?Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.CAUTION:?ABI does not recommend storage of dilutions of quantitative DNA Controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to “lose” copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.
Safe Handling Recommendation:?The DNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
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FAO（常見問題）：
?How are Quantitated DNA controls supplied??Quantitated DNA controls are supplied at 1-2 × 104?copies/μL (with the exception of BK and? JC polyomaviruses and PCV2, which are supplied at 1-2 × 105?copies/μL).? The copy number of? Quantitated DNA controls is determined by real-time PCR.
How can dilutions of Quantitated DNA controls be stored??ABI does not recommend storing dilutions of these controls under any conditions.? All? dilutions should be made immediately before use and used promptly.? We have observed that? dilutions used for standard curves typically “lose” copy number with time — sometimes as soon? as an hour or so after dilution.? This is especially common at dilutions less than 100-1000 copies? per microliter.
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Applications for use（適用范圍）:
- PCR
- Nucleic Acid-Based Assay
Quality control testing includes（質量控制檢測包括）:
- PCR
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Certificate of Analysis（分析報告）:
?點擊下載
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Reference Articles（已發表參考文獻）:
Stevens MP, Twin J, Fairley CK, et al. “Development and Evaluation of an ompA Quantitative Real-Time PCR Assay for Chlamydia trachomatis Serovar Determination.” Journal of Clinical Microbiology. 2010;48(6):2060-2065.
Xie F, Hu Y, Magee LA, et al. “Chlamydia pneumoniae Infection in Preeclampsia.” Hypertension in Pregnancy. 2010;29(04):468-477.
Cragnolini JJ, Garcia-Medel N, Lopez de Castro JA. “Endogenous Processing and Presentation of T-cell Epitopes from Chlamydia trachomatis with Relevance in HLA-B27-associated Reactive Arthritis.” Molecular & Cellular Proteomics. 2009;8(8):1850-1859.
Chen C-Y, Chi K-H, Alexander S, et al. “The Molecular Diagnosis of Lymphogranuloma Venereum: Evaluation of a Real-Time Multiplex Polymerase Chain Reaction Test Using Rectal and Urethral Specimens.” Sexually Transmitted Diseases. 2006;PAP
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